Acetylcholinesterase Antikörper (C-Term)

Produktnummer ABIN374526

Der Ziege Polyklonal anti-Acetylcholinesterase Antikörper (ABIN374526) detektiert spezifisch Acetylcholinesterase in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für Acetylcholinesterase Antikörper (C-Term) (ABIN374526)

Target: Acetylcholinesterase (AChE)

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Acetylcholinesterase Antikörper

Bindungsspezifität: C-Term

Sequenz: QFDHYSKQDR CSDL

Spezifität: This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000656.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetylcholinesterase

Hintergrund: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase

Gen-ID: 43, 9606

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development