KPNA3 Antikörper (C-Term)

Produktnummer ABIN374393

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Ziege Polyklonal anti-KPNA3 Antikörper wird verwendet zum Nachweis von KPNA3 in Proben von Human, Maus, Ratte, Rind (Kuh) und Hund. Er wurde validiert für WB, IP, IF, IHC (p) und EIA.

Kurzübersicht für KPNA3 Antikörper (C-Term) (ABIN374393)

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KPNA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-KPNA3 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-DPTANLQTKE FNF

Spezifität: This antibody detects Importin subunit alpha-3 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002258.2 Genename: KPNA3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.5 μg/mL. Approx 55-60 kDa band observed in Human Testis and Kidneylysates (predicted size of approx.50 kDa according to NP_002258). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Heart shows strong pixulate staining ofmyocardial fibres in transverse section.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-KPNA3 Antikörper (ABIN374393)

Silva, Martins, Tavares, Morgado: "Inhibition of spinal 5-HT3R reverted diabetes-induced mechanical hypersensitivity in a GABAAR-mediated neurotransmission-dependent manner." in: Neuroscience, Vol. 304, pp. 228-39, (2016) (PubMed).

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Ohara, Hata, Yasuhara, Mehmood, Yoneda, Nakagawa, Yamashita: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Details zu KPNA3

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Andere Bezeichnung: KPNA3 / Importin alpha-3

Hintergrund: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that KPNA3 may be involved in the nuclear transport system. The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.Synonyms: Importin alpha Q2, Karyopherin subunit alpha-3, QIP2, SRP1-gamma

Gen-ID: 3839

NCBI Accession: NP_002258

UniProt: O00505

Pathways: Protein targeting to Nucleus