T Cell Receptor (TCR) V beta-1 Antikörper

Antigen: T Cell Receptor (TCR) V beta-1

Klonalität: Monoklonal (TCR2)

Wirt: Maus

Reaktivität: Huhn

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Funktionale Studien (Func), Immunpräzipitation (IP)

Produktnummer: ABIN371346

Produktbeschreibung

Produktmerkmale: Synonyms: TCR-Vbeta1, Vbeta-1, VB1, TCRA, TCRB, TCR-VB1, T Cell Receptor alpha/beta

Kreuzreaktivität: Huhn

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: TCR2

Beschreibung: The receptors on T cells consist of immunoglobulin like integral membrane glycoproteinscontaining 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has,external to the cell membrane, an N terminal variable domain and a C terminal constantdomain. T cell receptors recognise foreign antigens which have been processed as small peptidesand bound to major histocompatibility complex molecules at the surface of antigenpresenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chainor one delta and one gamma chain. In a single cell, the T cell receptor loci are rearrangedand expressed in the order delta, gamma, beta, and alpha. If both delta and gammarearrangements produce functional chains, the cell expresses delta and gamma. If not, thecell proceeds to rearrange the beta and alpha loci.

Spezifität: This antibody recognizes TCR alpha/beta (Vß1). It precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and50-kDa upon reduction) on chicken peripheral blood T cells. (Ref. 1)Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa fromTCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appearin the thymus subsequent to the appearance of gamma delta T cells. Thesesubpopulations, originally denoted as TCR2 and TCR3 (Ref. 1-3) arise sequentially in thethymus during ontogeny and are now known to represent two distinct Vbeta families,

Anwendungen

Applikationshinweise: Flow Cytometry. (Ref.1)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). (Ref.7)Immunoprecipitation. (Ref.1)In ovo suppression of the development of TCR-Vbeta 1. (Ref.5,6)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Reinigung: Purified

Buffer: 100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar