T Cell Receptor (TCR) V beta-1 Antikörper (FITC)

Antigen: T Cell Receptor (TCR) V beta-1

Klonalität: Monoklonal (TCR2)

Wirt: Maus

Reaktivität: Huhn

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN371345

Produktbeschreibung

Produktmerkmale: Synonyms: TCR-Vbeta1, Vbeta-1, VB1, TCRA, TCRB, TCR-VB1, T Cell Receptor alpha/beta

Kreuzreaktivität: Huhn

Isotyp: IgG1

Klon: TCR2

Beschreibung: The receptors on T cells consist of immunoglobulin like integral membrane glycoproteinscontaining 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has,external to the cell membrane, an N terminal variable domain and a C terminal constantdomain. T cell receptors recognise foreign antigens which have been processed as small peptidesand bound to major histocompatibility complex molecules at the surface of antigenpresenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chainor one delta and one gamma chain. In a single cell, the T cell receptor loci are rearrangedand expressed in the order delta, gamma, beta, and alpha. If both delta and gammarearrangements produce functional chains, the cell expresses delta and gamma. If not, thecell proceeds to rearrange the beta and alpha loci.

Spezifität: This antibody recognizes TCR alpha/beta (Vß1). It precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and50-kDa upon reduction) on chicken peripheral blood T cells. (Ref. 1)Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa fromTCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appearin the thymus subsequent to the appearance of gamma delta T cells. Thesesubpopulations, originally denoted as TCR2 and TCR3 (Ref. 1-3) arise sequentially in thethymus during ontogeny and are now known to represent two distinct Vbeta families,Vbeta1 and Vbeta2, respectively. (Ref. 4)This antibody reacts with approximately 40% of thymocytes, 40-50% of bloodmononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence

Anwendungen

Applikationshinweise: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Buffer: PBS containing 0.09% Sodium Azide as preservative.Label: Fluorescein Isothiocyanate Isomer 1 (FITC)

Lagerung: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar