B-Cells (Subset) Antikörper

Antigen: B-Cells (Subset)

Klonalität: Monoklonal (BB6-10A10)

Wirt: Maus

Reaktivität: Schwein

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS)

Produktnummer: ABIN371312

Produktbeschreibung

Produktmerkmale: Synonyms: B Cells, B Lymphocytes, B-Lymphocytes

Kreuzreaktivität: Schwein

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: BB6-10A10

Beschreibung: B cells are lymphocytes that are produced in the bone marrow and require bone marrowstromal cells and their cytokines for maturation. During its development, each B cellbecomes genetically programmed through a series of gene splicing reactions to producean antibody molecule with a unique specificity.

Spezifität: This antibody stains the following percentages of surface immunoglobulin (sIg) positivecells in porcine lymphoid tissues: 11-15% in peripheral blood, 22-23% in mesenteric lymphnodes, 79-87% in ileal Peyer's patches (ILPP), 6-17 in spleen, and 0% in thymus (Ref. 1). While identification of the antigen(s) that are found predominantly on ILPP B cells andwhich are recognized by BB6-10A10 is speculative, the antibody appears to react with animmature population of B cells in the follicles of ILPPs and with a subpopulation of sIglowB cells in lymph nodes. Species: Pig. Others not tested.

Anwendungen

Applikationshinweise: Flow Cytometry. (Ref.1-2)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections only). (Ref.1)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.5 mg/ml

Reinigung: Purified

Buffer: 100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar