Immunoglobulin E (IgE) (Fc specific) Antikörper

Antigen: Immunoglobulin E (IgE)

Epitope: Fc specific

Klonalität: Monoklonal (BSW17-NBS01)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Durchflusszytometrie (FACS), Funktionale Studien (Func)

Produktnummer: ABIN363708

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin E (IgE) (Fc)

Gen-ID: IGHE, chromosome 14q32.33

Immunogen: purified human myeloma IgE

Format: Affinity-purified, Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1 kappa

Klon: BSW17-NBS01

Beschreibung: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.

Spezifität: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Anwendungen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Konzentration: 1mg/ml

Reinheit: > 95%, tested by analytical Gel Permeation Chromatography

Reinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Lagerung: Store undiluted at 4°C. For long term storage, aliquot and store at -20°C

Forschungsgebiet: Allergie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

product purity: gel permeation chromatography (Superose 12/HR)

Target binding kinetics (Biacore

Publikationen

Publikationen: Knutti-Mueller, Stadler, Magnusson et al.: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).

Czech, Stadler, Schuopf et al.: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Rudolf, Furukawa, Miescher et al.: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Rudolf, Zuercher, Nechansky et al.: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).