Acetylated Lysine Antikörper

Antigen: Acetylated Lysine

Klonalität: Monoklonal (7F8)

Wirt: Maus

Reaktivität: Rind (Kuh), Vogel

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN361802

Produktbeschreibung

Immunogen: Acetylated KLH

Format: Protein G purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 7F8

Beschreibung: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Ne-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).

Spezifität: Detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues. Species cross-reactivity: Bovine, Avian. Not otherwise tested.

Anwendungen

Konzentration: 1.0mg/mL

Buffer: Protein G purified. Mouse immunoglobulin in PBS, pH 7.4, 0.09% sodium azide in 50% glycerol

Lagerung: -20°C, 1 year+, shipped on cold packs or ambient

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Acetylated Lysine (7F8), mouse backskin

Publikationen

Publikationen: Hughes: "Polyglutamine disease: acetyltransferases awry." in: Current biology : CB, Vol. 12, Issue 4, pp. R141-3, 2002 (PubMed).

Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Yang: "Lysine acetylation and the bromodomain: a new partnership for signaling." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 10, pp. 1076-87, 2004 (PubMed).

Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).