NMDA Receptor, NR2C Subunit Antikörper

Antigen: NMDA Receptor, NR2C Subunit

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunpräzipitation (IP)

Produktnummer: ABIN361398

Produktbeschreibung

Format: Affinity-purified

Beschreibung: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Anti-NMDA Receptor, NR2C Subunit Western blot of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor.

Spezifität: Specific for the ~140k NR2C subunit of the NMDA receptor. Also labels the ~180k NR2A and the ~180k NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.

Anwendungen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC, IF (frozen sections, unpublished observations): 1:1000 to 1:2000 IP: 3 µl per 200 µg lysate Quality Control: Western blots performed on each lot.

Reinigung: Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Lagerung: Store at -20 °C, stable for 1 year

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blots of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor.

Publikationen

Publikationen: Wang, Imamura, Ishibashi et al.: "The Reck tumor suppressor protein alleviates tissue damage and promotes functional recovery after transient cerebral ischemia in mice." in: Journal of neurochemistry, 2010 (PubMed).