ATP Synthase Subunit II B', Chloroplastic (AtpG) Antikörper

Antigen: ATP Synthase Subunit II B', Chloroplastic (AtpG)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii)

Applikation: Western Blot (WB)

Produktnummer: ABIN361270

Produktbeschreibung

Weitere Bezeichnung: ATPsynthase subunit II b', chloroplastic (AtpG)

Immunogen: KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpGQ0WMW8 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785

Format: Lyophilized

Beschreibung: ATP synthase produces ATP from ADP in the presence of a proton gradientacross the membrane. F-type ATPases have two components, CF(1) - thecatalytic core - and CF(0) - the membrane proton channel. CF(1) has fivesubunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three mainsubunits: a, b and c. Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'

Spezifität: Predicted reactivity: dicots including: Sorghum bicolor, monocots including: Oryza sativa, Zea mays,algae Volvox carteri.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 10 000 (WB).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1),Chlamydomonas reinhardtii subunit II deletion mutant thylakoid membrane fraction (2),Arabidospsis thaliana thylakoid membrane fraction (3), were separated on 12-18%acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 hwith 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody(AS09 457, 1: 10 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000,1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation.Signal was detected with standard ECL (GE Healthcare), exposure time was 1 min. Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) Anovel pathway of cytochrome c biogenesis is involved in the assembly of the cytochromeb6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 andChlamydomonas membranes were prepared according to Chua & Bennoun (1975)Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutantstrains deficient in photosystem II reaction center. PNAS 72:2175-2179 Courtesy Dr. Yves Choquet 1