Der Kaninchen Polyklonal anti-SERPINA1 Antikörper wird verwendet zum Nachweis von SERPINA1 in Proben von Human. Er wurde validiert für WB, IHC (p) und EIA.
Suitable for most immunological methods. This product has been assayed against 1.0 μg of Human Alpha-1-anti-Trypsin in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L]MX and TMB as a substrate for 30 minutes at room temperature. A working dilution of1: 10,000 to 1: 50,000 is suggested for this product. Recommended Dilutions: ELISA : 1/10,000-1/600,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.41 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Target
SERPINA1
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))
Andere Bezeichnung
alpha-1-Antitrypsin
Hintergrund
Alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase.Synonyms: AAT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, SERPINA1