NBL1 Antikörper

Produktnummer ABIN360878

Der Ratte Monoklonal anti-NBL1 Antikörper wird verwendet zum Nachweis von NBL1 in Proben von Maus. Er wurde validiert für WB.

Kurzübersicht für NBL1 Antikörper (ABIN360878)

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser NBL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 7K12

Produktdetails anti-NBL1 Antikörper

Spezifität: This antibody detects Mouse DAN in Western blotting.

Aufreinigung: Protein A/G affinity chromatography

Immunogen: Recombinant DAN

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.2 mL sterile PBS and the final concentration is 0.5 mg/mL.

Buffer: 0.2 μm filtered PBS, None

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NBL1

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Andere Bezeichnung: NBL1

Hintergrund: DAN (differential screening-selected gene aberrative in neuroblastoma) was initially identified as a gene whose expression is downregulated in srctransformed rat fibroblasts. DAN has now been shown to be a prototypical member of the DAN family of secreted glycoproteins that are putative BMP antagonists. DAN family members share a cysteinerich domain that is structurally related to the cysteineknot motif found in TGFβ superfamily ligands. There are at least five mouse DAN family members, including DAN, Gremlin/DRM, Cer1 (Cerberusrelated), Dante and PRDC (protein-related to DAN and cerberus). Additional DAN family members include Xenopus Cerberus, chick Caronte, and C. elegans CeCan 1. Murine DAN binds BMP2 in immunoprecipitation experiments and acts as a BMP antagonist in Xenopus animal cap explants. The DAN family of proteins are thought to act as antagonists by binding BMP ligands and preventing their interactions with signaling receptor complexes. Recombinant mouse DAN have been shown to bind BMP4 in a functional ELISA. It is likely the various DAN family members and other TGFβ BMP antagonists including Noggin, Chordin, Follistatin, and TSG can selectively antagonize the activities of different subsets of TGFβ superfamily ligands. These antagonists represent one of the many elaborate regulatory mechanisms that have evolved to control the bioactivities of the TGFβ superfamily ligands.Synonyms: DAN, DAND1, Neuroblastoma suppressor of tumorigenicity 1

Gen-ID: 17965

NCBI Accession: NP_032701

UniProt: Q61477