AKR1C4 Antikörper (AA 2-14)

Produktnummer ABIN351481

Dieser Ziege Polyklonal Antikörper detektiert spezifisch AKR1C4 in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für AKR1C4 Antikörper (AA 2-14) (ABIN351481)

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AKR1C4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AKR1C4 Antikörper

Bindungsspezifität: AA 2-14

Spezifität: Human AKR1C4. This antibody may cross-react with AKR1C1

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Bovine (92%) Marmoset, Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide DPKYQRVELNDGH-C from the N-terminus of human AKR1C4 (NP_001809.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Bovine (92%), Marmoset, Horse (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:4000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu AKR1C4

Target: AKR1C4 (Aldo-keto Reductase Family 1 Member C4 (AKR1C4))

Andere Bezeichnung: AKR1C4 / Chlordecone Reductase

Hintergrund: Name/Gene ID: AKR1C4

Synonyms: AKR1C4, 3-alpha-HSD, 3-alpha-HSD1, Chlordecone reductase, CDR, CHDR, DD-4, Dihydrodiol dehydrogenase 4, C11, DD4, HAKRA

Gen-ID: 1109

NCBI Accession: NP_001809

UniProt: P17516

Pathways: Steroid Hormone Biosynthesis