NIF3L1 Antikörper (AA 365-377)

Produktnummer ABIN351470

Der Ziege Polyklonal anti-NIF3L1 Antikörper wird verwendet zum Nachweis von NIF3L1 in Proben von Human und Affe. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für NIF3L1 Antikörper (AA 365-377) (ABIN351470)

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NIF3L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NIF3L1 Antikörper

Bindungsspezifität: AA 365-377

Spezifität: Human NIF3L1. This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1, NP_068596.2) encode the same isoform.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%) Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%) Goat, Elephant, Bovine (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-ILSETDRDPLQVV from the C-terminus of human NIF3L1 (NP_001129511.1, NP_068596.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Opossum (100%), Mouse, Rat, Hamster, Panda, Bat, Dog, Horse, Turkey, Chicken (92%), Goat, Elephant, Bovine (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NIF3L1

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Andere Bezeichnung: ALS2CR1 / NIF3L1

Hintergrund: Name/Gene ID: NIF3L1

Synonyms: NIF3L1, CALS-7, MDS015, NIF3-like protein 1, ALS2CR1

Gen-ID: 60491

NCBI Accession: NP_001129511, NP_068596

UniProt: Q9GZT8