AKR1A1 Antikörper (AA 313-325)

Produktnummer ABIN351467

Der Ziege Polyklonal anti-AKR1A1 Antikörper wird verwendet zum Nachweis von AKR1A1 in Proben von Human, Maus, Ratte, Schwein, Rind (Kuh), Hund, Pferd, Kaninchen, Affe, Fledermaus, Hamster und Xenopus laevis. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für AKR1A1 Antikörper (AA 313-325) (ABIN351467)

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Hund, Pferd, Kaninchen, Affe, Fledermaus, Hamster, Xenopus laevis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AKR1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AKR1A1 Antikörper

Bindungsspezifität: AA 313-325

Spezifität: Human AKR1A1. Both reported variants (NP_006057.1and NP_697021.1) represent identical protein

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Xenopus (100%) Turkey, Chicken, Platypus, Catfish, Zebrafish (92%) Opossum, Salmon, Stickleback, Pike (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DAGHPLYPFNDPY from the C-terminus of human AKR1A1 (NP_006057.1, NP_697021.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Xenopus (100%), Turkey, Chicken, Platypus, Catfish, Zebrafish (92%), Opossum, Salmon, Stickleback, Pike (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu AKR1A1

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Andere Bezeichnung: AKR1A1

Hintergrund: Name/Gene ID: AKR1A1

Synonyms: AKR1A1, ALDR1, Aldehyde reductase, Alcohol dehydrogenase, ARM, DD3, Dihydrodiol dehydrogenase 3, ALR

Gen-ID: 10327

NCBI Accession: NP_006057, NP_697021

UniProt: P14550

Pathways: Monocarboxylic Acid Catabolic Process