ATF7 Antikörper (AA 482-494)

Produktnummer ABIN351459

Der Ziege Polyklonal anti-ATF7 Antikörper (ABIN351459) detektiert spezifisch ATF7 in WB, IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Pferd, Kaninchen, Fledermaus, Affe und Hamster.

Kurzübersicht für ATF7 Antikörper (AA 482-494) (ABIN351459)

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Pferd, Kaninchen, Fledermaus, Affe, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ATF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATF7 Antikörper

Bindungsspezifität: AA 482-494

Spezifität: Human ATF7. This antibody is expected to recognise all three reported isoforms (NP_001123531.1, NP_006847.1 and NP_001123532.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Lizard (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-HVIMTPQSQSAGR from the C-terminus of human ATF7 (NP_001123531.1, NP_006847.1, NP_001123532.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%), Lizard (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ATF7

Target: ATF7 (Activating Transcription Factor 7 (ATF7))

Andere Bezeichnung: ATF7

Hintergrund: Name/Gene ID: ATF7
Family: Transcription factor

Synonyms: ATF7, ATFA, Transcription factor ATF-A

Gen-ID: 11016

NCBI Accession: NP_001123531, NP_006847, NP_001123532

UniProt: P17544