Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3) Antikörper

Antigen: Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349671

Produktbeschreibung

Weitere Bezeichnung: PIP1 (PIP1,1, PIP1,2, PIP1,3) aquaporins

Immunogen: synthetic peptide conserved in Arabidopsis thaliana: PIP1,1 P61837, PIP1,2Q06611, PIP1,3 Q08733, PIP1,4 Q39196

Format: Serum

Beschreibung: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2,PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,

Spezifität: Predicted reactivity: dicots including: Brassica sp., Vicia faba, monocots: Hordeum vulgare, Oryzasativa, trees: Juglans regia, Populus tremula.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIPs antibodies (ABIN349671, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publikationen

Publikationen: Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).