Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 1 (SLC9A1) Antikörper

Antigen: Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 1 (SLC9A1)

Synonyme: APNH, NHE1, FLJ42224, swe, Apnh, Nhe1, AW554487, SLC9A1, AT-NHX1, ATNHX, ATNHX1, NA(+)/H(+) ANTIPORTER, Na+/H+ exchanger 1, NHX1, T21B4.60, T21B4_60

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349668

Produktbeschreibung

Weitere Bezeichnung: Na+/H+ antiporter, sodium/hydrogen exchanger (NHE-1)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX proteinQ68KI4

Format: Serum

Beschreibung: Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cationssuch as sodium and potassium across membraes. Localized in tonoplast,possibily also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1

Spezifität: Predicted reactivity: dictos including: Gossypium hirsutum, Ricinus communis, Vitis vinifera, monocotsincluding: Oryza sativa, Zea mays, trees: Populus euphratica.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 1000 with standard ECL (WB), 1: 8000 (ELISA).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Protocol for vacuolar membrane isolation can be found here.

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana (left panel) and 1,5 and 10ug of crude membrane fraction/lane Raphanussativus L. (right panel) were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters wereblocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100).Membranes were washed 5 times with TBS-T, each time in a fresh polystyrenebox and probed with anti-NHX antibodies (ABIN349668, 1:1000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publikationen

Publikationen: Chen, Xiao, Wu et al.: "Nitric oxide enhances salt secretion and Na(+) sequestration in a mangrove plant, Avicennia marina, through increasing the expression of H(+)-ATPase and Na(+)/H(+) antiporter under high salinity." in: Tree physiology, Vol. 30, Issue 12, pp. 1570-85, 2010 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (1:2000).