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Biotin Antikörper
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| Klonalität |
Polyklonal |
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Wirt
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Applikation
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Alternativen ELISA, Western Blot (WB), Immunhistochemie (IHC)
Western Blot (WB) (82), ELISA (63), Durchflusszytometrie (FACS) (57), Immunfluoreszenz (IF) (45), Immunpräzipitation (IP) (37), Enzyme Immunoassay (EIA) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (30), Immunhistochemie (IHC) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (14), Dot Blot (Dot) (8), Immunodiffusion (ID) (6), Immunzytochemie (ICC) (3), Immunelektrophorese (IEP) (1), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN349590 |
| Menge |
1.0 mg (2.55 mg/ml (by UV absorbance at 280...) (Varianten) |
| Preis |
159,34 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 bis 7 Werktagen |
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Immunogen
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Biotin conjugated to Keyhole Limpet Hemocyanin (KLH) Conjugation
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Format
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Liquid (sterile filtered)
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Spezifität
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and anti-Fluorescein. No reaction was observed against anti-papain or anti-Goat IgG F(c).
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Applikationshinweise
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Recommended Dilutions: ELISA 1:36,000 WESTERN BLOT User Optimized IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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Konzentration
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2.55 mg/ml (by UV absorbance at 280 nm)
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Buffer
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Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
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Lagerung
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Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
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Forschungsgebiet
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Signaltransduktion, Metabolismus, Epitop-Tags, Tags/Labels
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Biotin", Typ "Antikörper" finden
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Wirte
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Ziege (104), Maus (30), Kaninchen (30), Schaf (3)
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Reaktivitäten
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Alle Spezies (1), Human (1)
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Applikationen
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Western Blot (WB) (82), ELISA (63), Durchflusszytometrie (FACS) (57), Immunfluoreszenz (IF) (45), Immunpräzipitation (IP) (37), Enzyme Immunoassay (EIA) (36), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (30), Immunhistochemie (IHC) (23), Immunhistochemie (Paraffinschnitte) (IHC (p)) (14), Dot Blot (Dot) (8), Immunodiffusion (ID) (6), Immunzytochemie (ICC) (3), Immunelektrophorese (IEP) (1), Radioimmunoassay (RIA) (1)
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Konjugate
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FITC (21), HRP (20), Alkaline Phosphatase (AP) (10), Texas Red (TR) (10), Cy2 (5), Cy5.5 (4), PE (4), Cy3.5 (3), Biotin (2), Colloidal Gold (15 OD) (2), Cy5 (2), Rhodamine (2), TRITC (2), 488-NHS Ester (1), DTAF (1), Peroxidase (POD) (1)
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