AKR1C3 Antikörper (AA 311-323)

Produktnummer ABIN343889

Der Ziege Polyklonal anti-AKR1C3 Antikörper wird verwendet zum Nachweis von AKR1C3 in Proben von Human. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für AKR1C3 Antikörper (AA 311-323) (ABIN343889)

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AKR1C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-AKR1C3 Antikörper

Bindungsspezifität: AA 311-323

Spezifität: Human AKR1C3.

Homologie: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Orangutan (92%), Gibbon (92%), Monkey (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-FASHPNYPYSDEY from the C-terminus of human AKR1C3 (NP_003730.4). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Orangutan (92%), Gibbon (92%), Monkey (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.01 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu AKR1C3

Target: AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))

Andere Bezeichnung: DDX / AKR1C3

Hintergrund: Name/Gene ID: AKR1C3

Synonyms: AKR1C3, 3-alpha-HSD type 2, 17-beta-HSD 5, 3-alpha-HSD type II, brain, DD3, DDH1, DDX, Dihydrodiol dehydrogenase 3, Dihydrodiol dehydrogenase X, DD-3, HA1753, HAKRe, HAKRB, HSD17B5, KIAA0119, Kia0119, Prostaglandin F synthase, PGFS, Truncated aldoketo reductase a, HluPGFS, Indanol dehydrogenase

Gen-ID: 8644

NCBI Accession: NP_003730

UniProt: P42330

Pathways: Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus