Gene Transfer Agent (GTA) Major Capsid Protein (MCP) (GTA MCP ) Antikörper

Antigen: Gene Transfer Agent (GTA) Major Capsid Protein (MCP) (GTA MCP )

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Rhodobacter capsulatus, Ruegeria (Silicibacter) pomeroyi DSS-3

Applikation: Western Blot (WB)

Produktnummer: ABIN343850

Produktbeschreibung

Immunogen: KLH-conjugated conserved peptide sequence found in the Gene Transfer Agent(GTA) major capsid protein (MCP) encoded in Bacteria within theRhodobacterales order of the class alpha-Proteobacteria including RhodobactersphaeroidesQ3J3K4

Format: Lyophilized

Beschreibung: Gene Transfer Agents (GTAs) are bacteriophage-like particles which function as a means to package and transfer genomic DNA between bacterial cells. GTA-mediated gene transfer might be an important phenomenon in naturalmicrobial communities.

Spezifität: Predicted reactivity: Rhodobacterales.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 1000 (WB).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Bakterien

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Roseobacter capsulatus cells, pelleted by centrifugation and resuspended in an equial volume of TE buffer and supernatant sample* were separated on 10% SDS-PAGE and blotted 1h to nitrocellulose. The "+" indicated R. capsulatus SB1003 (GTA positive) and "-" indicated R. capsulatus A1 (GTA capsid protein negative). Blots were blocked in 5% skim milk in TBST followed by incubation with anti-GTA antibodies (AS08 365) at dilution 1: 1000 at 4