CD9 (CD9) Antikörper
| Antigen |
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| Synonyme |
5H9, BA2, P24, GIG2, MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29 |
| Klonalität |
Monoklonal (EM-04) |
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Wirt
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Reaktivität
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Human (158), Maus (44), Rind (Kuh) (25), Katze (21), Hund (21), Pferd (19), Kaninchen (17), Affe (12), Ratte (Rattus) (10), Schwein (7), Schaf (4)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)
Durchflusszytometrie (FACS) (178), Western Blot (WB) (73), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (43), Immunpräzipitation (IP) (39), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Funktionale Studien (Func) (18), Immunfluoreszenz (IF) (17), Immunhistochemie (IHC) (12), Enzyme Immunoassay (EIA) (7), ELISA (5), Immunzytochemie (ICC) (3), Blockierende Antikörper (Inhibition) (1), Dot Blot (Dot) (1)
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| Produktnummer |
ABIN343715 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
140,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD9
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Immunogen
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Permeabilized murine bone marrow-derived mast cells (BMMC).
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Format
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Purified
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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EM-04
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Beschreibung
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CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associatedtetraspanin-enriched signaling microdomains within the plasma membrane, formingcomplexes with each other as well as with integrins, membrane-anchored growth factorsand other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth,myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferationand is necessary for association of heterologous MHC II molecules on the dendritic cellplasma membrane which is important for effective T cell stimulation. CD9 is also consideredas metastasis suppressor in solid tumors.
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Spezifität
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The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembraneprotein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activatedT lymphocytes.
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Applikationshinweise
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Flow Cytometry: Recommended dilution: 4 µg/ml. - Recommended dilution: 1 µg/ml. Application note: non-reducing conditions
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from hybridoma culture supernatant by hydrophobic charge inductionchromatography followed by precipitation methods.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Embryogenese, Embryonale Stammzellen, Reproduktion, CD Antigene, Oberflächenrezeptoren der Immunzellen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "CD9 (CD9)", Typ "Antikörper" finden
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Wirte
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Maus (157), Ratte (32), Kaninchen (24)
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Reaktivitäten
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Human (158), Maus (44), Rind (Kuh) (25), Katze (21), Hund (21), Pferd (19), Kaninchen (17), Affe (12), Ratte (Rattus) (10), Schwein (7), Schaf (4)
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Applikationen
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Durchflusszytometrie (FACS) (178), Western Blot (WB) (73), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (43), Immunpräzipitation (IP) (39), Immunhistochemie (Paraffinschnitte) (IHC (p)) (36), Funktionale Studien (Func) (18), Immunfluoreszenz (IF) (17), Immunhistochemie (IHC) (12), Enzyme Immunoassay (EIA) (7), ELISA (5), Immunzytochemie (ICC) (3), Blockierende Antikörper (Inhibition) (1), Dot Blot (Dot) (1)
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Konjugate
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PE (33), FITC (32), Biotin (17), APC (4), RPE (3), Alexa Fluor 647 (2), Alexa Fluor 488 (1), Alexa Fluor 700 (1), Pacific Blue (1)
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Epitope
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N-Term (3), Center (2)
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