AGR2+AGR3 Antikörper

Antigen: AGR2+AGR3

Klonalität: Monoklonal (AGR3-4)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN343713

Produktbeschreibung

Immunogen: Purified human AGR3 protein

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: AGR3-4

Beschreibung: AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, andAGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, aresecreted cytoplasmic proteins which are involved in metastasis induction and p53 tumoursupressor inhibition. They may serve as molecular markers and potential therapeutic targetsfor hormone-responsive breast tumours, AGR2 was reported also as a marker of othercarcinomas. Xenopus homolog of these proteins is associated with anteroposterior fatedetermination during early development.

Spezifität: The antibody AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2)proteins (19-20 kDa), secreted cytoplasmic proteins which can serve as markers ofcarcinogenesis.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line. Immunocytochemistry - Recommended dilution: 1 µg/ml. Positive control: T47D breast cancer cell line. Negative control: H1299 lung carcinoma cell line

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.