MIF Antikörper (AA 103-115)

Produktnummer ABIN337308

Der Ziege Polyklonal anti-MIF Antikörper (ABIN337308) detektiert spezifisch MIF in WB, IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Affe.

Kurzübersicht für MIF Antikörper (AA 103-115) (ABIN337308)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MIF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MIF Antikörper

Bindungsspezifität: AA 103-115

Spezifität: Human MIF.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%) Hamster (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NAANVGWNNSTFA from the C-terminus of human MIF (NP_002406.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Andere Bezeichnung: MIF

Hintergrund: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

Gen-ID: 4282

NCBI Accession: NP_002406

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling