PEDF Antikörper (N-Term)

Produktnummer ABIN6739671

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PEDF in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Hund, Meerschweinchen, Pferd, Schaf und Fledermaus.

Kurzübersicht für PEDF Antikörper (N-Term) (ABIN6739671)

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reaktivität: Human, Maus, Ratte, Schwein, Hund, Meerschweinchen, Pferd, Schaf, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PEDF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PEDF Antikörper

Bindungsspezifität: N-Term

Spezifität: Human SERPINF1 / PEDF

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Sheep (86%) Horse, Pig, Guinea pig (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SERPINF1 (P36955, NP_002606). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Bat (100%), Gibbon (93%), Galago (92%), Marmoset, Mouse, Sheep, Bovine (86%), Opossum, Platypus (81%), Elephant, Horse, Pig, Guinea pig (80%), Rat (75%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (4 - 8 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4-8 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Andere Bezeichnung: SERPINF1 / PEDF

Hintergrund: Name/Gene ID: SERPINF1

Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

Gen-ID: 5176

NCBI Accession: NP_002606

UniProt: P36955