ALDH1A1 Antikörper (AA 476-488)

Produktnummer ABIN337223

Der Ziege Polyklonal anti-ALDH1A1 Antikörper wird verwendet zum Nachweis von ALDH1A1 in Proben von Human, Rind (Kuh) und Schaf. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für ALDH1A1 Antikörper (AA 476-488) (ABIN337223)

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reaktivität: Human, Rind (Kuh), Schaf

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ALDH1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ALDH1A1 Antikörper

Bindungsspezifität: AA 476-488

Spezifität: Human ALDH1A1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%) Marmoset, Bat, Horse, Opossum, Lizard (92%) Dog, Chicken, Platypus, Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-RELGEYGFHEYTE from an internal region of human ALDH1A1 (NP_000680.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Sheep, Bovine (100%), Marmoset, Bat, Horse, Opossum, Lizard (92%), Dog, Chicken, Platypus, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Andere Bezeichnung: ALDH1A1 / ALDH1

Hintergrund: Name/Gene ID: ALDH1A1

Synonyms: ALDH1A1, Acetaldehyde dehydrogenase 1, ALDH class 1, ALDC, Aldehyde dehydrogenase 1, ALDH-E1, ALDH1, ALDH11, PUMB1, RALDH 1, RALDH1, ALHDII, Retinal dehydrogenase 1, Retinaldehyde dehydrogenase 1

Gen-ID: 216

NCBI Accession: NP_000680

UniProt: P00352

Pathways: Dopaminergic Neurogenesis