FHL1 Antikörper (AA 261-272)

Produktnummer ABIN337221

Der Ziege Polyklonal anti-FHL1 Antikörper (ABIN337221) detektiert spezifisch FHL1 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hamster.

Kurzübersicht für FHL1 Antikörper (AA 261-272) (ABIN337221)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reaktivität: Human, Maus, Ratte, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FHL1 Antikörper

Bindungsspezifität: AA 261-272

Spezifität: Human FHL1. This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Homologie: Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%) Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CNKRFVFHNEQVY from an internal region of human FHL1 (NP_001440.1, NP_001153171.1). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%), Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Xenopus (92%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.05 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Andere Bezeichnung: SLIM / FHL1

Hintergrund: Name/Gene ID: FHL1

Synonyms: FHL1, BA535K18.1, FHL1B, FHL1A, FLH1A, Four and a half LIM domains 1, FHL-1, KYOT, Skeletal muscle LIM-protein 1, SLIM-1, Skeletal muscle LIM-protein1, SLIMMER, XMPMA, KYO-T, LIM protein SLIMMER, SLIM, SLIM1

Gen-ID: 2273

NCBI Accession: NP_001440, NP_001153171

UniProt: Q13642