PDLIM1 Antikörper (AA 316-329)

Produktnummer ABIN337145

Der Ziege Polyklonal anti-PDLIM1 Antikörper wird verwendet zum Nachweis von PDLIM1 in Proben von Human. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für PDLIM1 Antikörper (AA 316-329) (ABIN337145)

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PDLIM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDLIM1 Antikörper

Bindungsspezifität: AA 316-329

Spezifität: Human PDLIM1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Rat, Hamster, Panda, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Zebrafish (93%) Elephant, Bovine, Pig, Stickleback (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-TPPEGYEVVTVFPK from the C-terminus of human PDLIM1 (NP_066272.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Rat, Hamster, Panda, Bat, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Zebrafish (93%), Elephant, Bovine, Pig, Stickleback (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PDLIM1

Target: PDLIM1 (PDZ and LIM Domain 1 (PDLIM1))

Andere Bezeichnung: PDLIM1

Hintergrund: Name/Gene ID: PDLIM1

Synonyms: PDLIM1, CLP-36, CLP36, CLIM1, Elfin, ENIGMA, HCLIM1, PDZ and LIM domain 1, Lim, PDZ and LIM domain 1 (elfin), PDZ and LIM domain protein 1, LIM domain protein CLP-36

Gen-ID: 9124

NCBI Accession: NP_066272

UniProt: O00151