CES1 Antikörper (AA 533-544)

Produktnummer ABIN337128

Der Ziege Polyklonal anti-CES1 Antikörper (ABIN337128) detektiert spezifisch CES1 in WB, IHC, ELISA und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CES1 Antikörper (AA 533-544) (ABIN337128)

Target: CES1 (Carboxylesterase 1 (CES1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CES1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CES1 Antikörper

Bindungsspezifität: AA 533-544

Spezifität: Human CES1. This antibody is expected to recognise all three reported isoforms (NP_001020366.1, NP_001020365.1, NP_001257.4).

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Monkey (100%) Gorilla, Gibbon, Marmoset, Horse (92%) Mouse, Rat, Hamster, Panda, Bovine, Cat (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NTQAAQKLKDKE from an internal region (near C-terminus) of human CES1 (NP_001020366.1, NP_001020365.1, NP_001257.4). Percent identity by BLAST analysis: Human, Orangutan, Monkey (100%), Gorilla, Gibbon, Marmoset, Horse (92%), Mouse, Rat, Hamster, Panda, Bovine, Cat (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:8000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Andere Bezeichnung: Carboxylesterase 1 / CES1

Hintergrund: Name/Gene ID: CES1

Synonyms: CES1, ACAT, Brain carboxylesterase hBr1, Carboxylesterase 1, Carboxylesterase 2 (liver), CEH, CES1A1, CES1A2, Cocaine carboxylesterase, Egasyn, HMSE, Liver carboxylesterase 1, HCE-1, PCE-1, Retinyl ester hydrolase, TGH, Triacylglycerol hydrolase, REH, SES1, CE-1, Cholesteryl ester hydrolase, HMSE1, Serine esterase 1

Gen-ID: 1066

NCBI Accession: NP_001020366, NP_001020365, NP_001257

UniProt: P23141

Pathways: Monocarboxylic Acid Catabolic Process