SNAP25 Antikörper (AA 193-206)

Produktnummer ABIN337119

Der Ziege Polyklonal anti-SNAP25 Antikörper (ABIN337119) detektiert spezifisch SNAP25 in WB, ELISA, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Hund, Rind (Kuh), Huhn, Zebrafisch (Danio rerio), Affe, Hamster, Pferd, Kaninchen und Fledermaus.

Kurzübersicht für SNAP25 Antikörper (AA 193-206) (ABIN337119)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Huhn, Zebrafisch (Danio rerio), Affe, Hamster, Pferd, Kaninchen, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SNAP25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SNAP25 Antikörper

Bindungsspezifität: AA 193-206

Spezifität: Human SNAP25. This antibody is expected to recognise both reported isoforms NP_003072 and NP_570824.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%) Frog (93%) Xenopus (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DEANQRATKMLGSG from the C-terminus of human SNAP25 (NP_003072.2, NP_570824.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Chicken, Platypus, Lizard, Newt, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish (100%), Frog (93%), Xenopus (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Andere Bezeichnung: SNAP25

Hintergrund: Name/Gene ID: SNAP25
Family: SNAP-25

Synonyms: SNAP25, BA416N4.2, DJ1068F16.2, SNAP, SUP, Super protein, RIC4, SEC9, SNAP-25, RIC-4

Gen-ID: 6616

NCBI Accession: NP_003072, NP_570824

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport