s100a4 Antikörper (AA 86-98)

Produktnummer ABIN337109

Der Ziege Polyklonal anti-s100a4 Antikörper wird verwendet zum Nachweis von s100a4 in Proben von Human, Rind (Kuh), Kaninchen, Hund, Pferd und Schwein. Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für s100a4 Antikörper (AA 86-98) (ABIN337109)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reaktivität: Human, Rind (Kuh), Kaninchen, Hund, Pferd, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser s100a4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-s100a4 Antikörper

Bindungsspezifität: AA 86-98

Spezifität: Human S100A4 / FSP1. Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Elephant, Bat (92%) Mouse, Rat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CNEFFEGFPDKQP from the C-terminus of human S100A4 / FSP1 (NP_002952.1, NP_062427.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Elephant, Bat (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4 / FSP1

Hintergrund: Name/Gene ID: S100A4

Synonyms: S100A4, 42A, 18A2, Fibroblast-specific protein-1, FSP1, MTS1, PEL98, Protein Mts1, Calvasculin, CAPL, Metastasin, p9KA, Protein S100-A4

Gen-ID: 6275

NCBI Accession: NP_002952, NP_062427

UniProt: P26447

Pathways: S100 Proteine