Merlin Antikörper (AA 526-536)

Produktnummer ABIN337064

Der Ziege Polyklonal anti-Merlin Antikörper wird verwendet zum Nachweis von Merlin in Proben von Human und Zebrafisch (Danio rerio). Er wurde validiert für ELISA, IHC und IHC (p).

Kurzübersicht für Merlin Antikörper (AA 526-536) (ABIN337064)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Merlin Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Merlin Antikörper

Bindungsspezifität: AA 526-536

Spezifität: Zebrafish NF2.

Homologie: Percent identity by BLAST analysis: Pufferfish, Zebrafish (100%) Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Drosophila, Ant, Paramecium (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKSKHLQDQLK from an internal region of zebrafish NF2 (NP_998116.1). Percent identity by BLAST analysis: Pufferfish, Zebrafish (100%), Human, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Drosophila, Ant, Paramecium (82%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Zebrafish

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Andere Bezeichnung: NF2 / Merlin

Hintergrund: Name/Gene ID: NF2

Synonyms: NF2, ACN, BANF, Moesin-ezrin-radixin like, Neurofibromin 2 (merlin), Neurofibromin-2, SCH, Merlin, Schwannomerlin, Schwannomin

Gen-ID: 4771

NCBI Accession: NP_998116

UniProt: P35240

Pathways: Cell-Cell Junction Organization