EIF4ENIF1 Antikörper (AA 974-986)

Produktnummer ABIN337018

Dieser Ziege Polyklonal Antikörper detektiert spezifisch EIF4ENIF1 in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Schwein, Xenopus laevis, Hund, Pferd, Kaninchen und Fledermaus.

Kurzübersicht für EIF4ENIF1 Antikörper (AA 974-986) (ABIN337018)

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Reaktivität: Human, Schwein, Xenopus laevis, Hund, Pferd, Kaninchen, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EIF4ENIF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EIF4ENIF1 Antikörper

Bindungsspezifität: AA 974-986

Spezifität: Human EIF4ENIF1. This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2, NP_001157973.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Shrew, Bat, Dog, Elephant, Panda, Rabbit, Horse, Pig, Armadillo, Xenopus (100%) Marmoset, Mouse, Rat, Bovine, Hamster, Opossum, Turkey, Chicken, Platypus (92%) Medaka, Zebrafish, Stickleback (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-AKVISVDELEYRQ from the C-terminus of human EIF4ENIF1 (NP_062817.2, NP_001157974.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Shrew, Bat, Dog, Elephant, Panda, Rabbit, Horse, Pig, Armadillo, Xenopus (100%), Marmoset, Mouse, Rat, Bovine, Hamster, Opossum, Turkey, Chicken, Platypus (92%), Medaka, Zebrafish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (0.25 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EIF4ENIF1

Target: EIF4ENIF1 (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1))

Andere Bezeichnung: EIF4ENIF1 / 4E-T

Hintergrund: Name/Gene ID: EIF4ENIF1

Synonyms: EIF4ENIF1, 4E-T, EIF4E transporter, EIF4E-transporter, Clast4

Gen-ID: 56478

NCBI Accession: NP_062817, NP_001157974