ACAP1 Antikörper (AA 728-740)

Produktnummer ABIN337010

Der Ziege Polyklonal anti-ACAP1 Antikörper wird verwendet zum Nachweis von ACAP1 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Affe und Kaninchen. Er wurde validiert für ELISA, WB, IHC und IHC (p).

Kurzübersicht für ACAP1 Antikörper (AA 728-740) (ABIN337010)

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Affe, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACAP1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ACAP1 Antikörper

Bindungsspezifität: AA 728-740

Spezifität: Human ACAP1 / CENTB1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%) Horse, Pig (92%) Bat (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-EKLSRRSHDLHTL from the C-terminus of human ACAP1 / CENTB1 (NP_055531.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Rabbit, Opossum (100%), Horse, Pig (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ACAP1

Target: ACAP1 (ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 1 (ACAP1))

Andere Bezeichnung: CENTB1 / ACAP1

Hintergrund: Name/Gene ID: ACAP1

Synonyms: ACAP1, Centaurin, beta 1, Centaurin-beta-1, Cnt-b1, KIAA0050, CENTB1

Gen-ID: 9744

NCBI Accession: NP_055531

UniProt: Q15027