S-100 Antikörper

Antigen: S-100

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Affe, Maus, Ratte (Rattus), Rind (Kuh)

Applikation: Immunhistochemie (IHC), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN336962

Produktbeschreibung

Weitere Bezeichnung: S100

Immunogen: Purified S100 protein

Format: Liquid (prediluted)

Beschreibung: S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix (EF-hand type) conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH. Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells. This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis.

Spezifität: Molecular Weight: ~22 kDa.

Anwendungen

Applikationshinweise: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:200 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. The optimum dilution should be determined by the individual lab. Cellular Localization: Cytoplasmic.

Buffer: :Immunized serum in10 mM PO4, 150 mM NaCl, pH 7.4, 0.05% sodium azide. HostRabbit Intended Use Immunohistochemistry (IHC) and Immunocytchemistry (ICC)

Lagerung: 2-8°

Forschungsgebiet: Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar