4-HNE Antikörper

Antigen: 4-HNE

Klonalität: Monoklonal (HNEJ-2)

Wirt: Maus

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB)

Produktnummer: ABIN335415

Produktbeschreibung

Immunogen: 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.

Format: Lyophilized

Isotyp: IgG1-kappa  (Passende Sekundärantikörper)

Klon: HNEJ-2

Beschreibung: During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.

Spezifität: Specific for 4HNE-Lysine adduct in all species. This antibody has almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.

Anwendungen

Applikationshinweise: IHC, Western and/or ELISA. Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.

Reinigung: Ammonium sulphate purified IgG

Buffer: Lyophilized as 100 ug/mL IgG in 50mM Tris buffered saline (TBS)

Lagerung: Less than -20°C, stable for 5 years Recinstitute with 200µl of distilled water.

Forschungsgebiet: Neurologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

Publikationen

Publikationen: Toyokuni, Miyake, Hiai et al.: "The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct." in: FEBS letters, Vol. 359, Issue 2-3, pp. 189-91, 1995 (PubMed).

Tanaka, Nishiyama, Okada et al.: "Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 77, Issue 2, pp. 145-55, 1997 (PubMed).

Nakajima, Inokuchi, Shimazawa et al.: "Astaxanthin, a dietary carotenoid, protects retinal cells against oxidative stress in-vitro and in mice in-vivo." in: The Journal of pharmacy and pharmacology, Vol. 60, Issue 10, pp. 1365-74, 2008 (PubMed).

Sedlak, Saleh, Higginson et al.: "Bilirubin and glutathione have complementary antioxidant and cytoprotective roles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 13, pp. 5171-6, 2009 (PubMed).

Boretti, Buehler, DAgnillo et al.: "Sequestration of extracellular hemoglobin within a haptoglobin complex decreases its hypertensive and oxidative effects in dogs and guinea pigs." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2271-80, 2009 (PubMed).