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Vimentin (VIM) Antikörper

Antigen

Vimentin (VIM)

Synonyme FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Klonalität Monoklonal (RV203)
Wirt
Reaktivität
Alternativen

Human, Ratte (Rattus), Ziege, Hund, Hamster, Schwein

Applikation
Alternativen Durchflusszytometrie (FACS), Immunzytochemie (ICC), Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)
Produktnummer ABIN335381
Menge 100ug  (Varianten)
Preis 418,18 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 3 bis 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung Vimentin
Immunogen RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Format Purified
Isotyp IgG1  (Passende Sekundärantikörper)
Klon RV203
Beschreibung Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Spezifität Human, rat, goat, canine, hamster and swine.

Anwendungen

Applikationshinweise RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Lagerung Store at 4 o C, or in small aliquots at -20 o C.
Forschungsgebiet Abstammungsmarker, Zytoskelett, Neuronale Stammzellmarker, Zell-/Gewebemarker
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "Vimentin (VIM)", Typ "Antikörper" finden
Wirte Kaninchen (116), Maus (106), Huhn (13), Ratte (6), Schaf (6), Ziege (2), Meerschweinchen (2)
Reaktivitäten Human (216), Ratte (Rattus) (94), Maus (86), Huhn (38), Rind (Kuh) (30), Schwein (27), Hund (10), Säugetiere (8), Hamster (6), Affe (6), Ziege (5), Amphibien (4), Potoroo (2), Katze (1), Fisch (1), Pferd (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
Applikationen Western Blot (WB) (161), Immunfluoreszenz (IF) (97), Immunhistochemie (Paraffinschnitte) (IHC (p)) (77), Durchflusszytometrie (FACS) (58), Immunhistochemie (IHC) (57), ELISA (43), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (42), Immunpräzipitation (IP) (26), Immunzytochemie (ICC) (25), Enzyme Immunoassay (EIA) (23), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2)
Konjugate FITC (10), Biotin (8), Alexa Fluor 488 (4), HRP (4), PE (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitope pSer82 (23), C-Term (2), Center (2), S82 (2), pSer33 (2), pSer38 (2), pSer50 (2), pSer55 (2), pSer6 (2), pSer71 (2), Ab-56 (1), Ab-83 (1), Fragment (1), pSer56 (1), pSer83 (1)