Desmin (DES) Antikörper

Antigen: Desmin (DES)

Synonyme: DES, des-a, MGC52614, des-b, MGC80853, CSM1, CSM2, CMD1I, FLJ12025, FLJ39719, FLJ41013, FLJ41793, des, csm1, csm2, desm, cmd1i, MGC75911, LOC395906, LOC100220724, desmin, cb290, fb59a12, MGC109859, wu:fb59a12, zgc:109859, MGC154009, wu:fc11d08

Klonalität: Monoklonal (RD301)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Kaninchen, Hamster, Hund, Huhn, Schwein

Applikation: Immunhistochemie (IHC), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)

Produktnummer: ABIN335360

Produktbeschreibung

Weitere Bezeichnung: Desmin

Immunogen: RD301 is a mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal desmin extract of chicken gizzard.

Format: Purified

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: RD301

Beschreibung: Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Spezifität: Human, mouse, rat, rabbit, hamster, canine, chicken and swine.

Synonyme: DES, des-a, MGC52614, des-b, MGC80853, CSM1, CSM2, CMD1I, FLJ12025, FLJ39719, FLJ41013, FLJ41793, des, csm1, csm2, desm, cmd1i, MGC75911, LOC395906, LOC100220724, desmin, cb290, fb59a12, MGC109859, wu:fb59a12, zgc:109859, MGC154009, wu:fc11d08

Anwendungen

Applikationshinweise: RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Lagerung: Store at 4 o C, or in small aliquots at -20 o C.

Forschungsgebiet: Krebs, Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Desmin (DES) antibody

anti-Desmin (DES) antibody (Image 2)

Publikationen

Publikationen: Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Verhagen, Aalders, Ramaekers et al.: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, 1988 (PubMed).

Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).

Schaart, Viebahn, Langmann et al.: "Desmin and titin expression in early postimplantation mouse embryos." in: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, 1990 (PubMed).

Quax, van den Broek, Egberts et al.: "Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer." in: Cell, Vol. 43, Issue 1, pp. 327-38, 1986 (PubMed).

Dispersyn, Geuens, Ver Donck et al.: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, 2001 (PubMed).