Nitrate reductase, assimilatory (NR) Antikörper

Antigen: Nitrate reductase, assimilatory (NR)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Red algae (Gracilaria), Thalassiosira

Applikation: Western Blot (WB)

Produktnummer: ABIN334562

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from conserved domain in NADH-NRprotein sequences including A.thaliana NR1 P11832 and NR2 P11035

Format: Lyophilized

Beschreibung: Assimilatory nitrate reductase (NR, EC.1.6.6.1) catalyses the reduction of nitrateto nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (mostcommon form in plants and algae, predominantly found in green tissues) andNAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutivelyexpressed in plants at a low level). NADH-NR is a homodimer of two identicalsubunits (100-115 kDa each, hold together by a Mo-cofactor) each of them codedby up to three genes (NR1-3, NIA1-NIA3).

Spezifität: Predicted reactivity: dicots including: Glycine max, Lycopersicum esculentum, Nicotiana tabacum,Ricinus communis, Solanum tuberosum, monocots including: Oryza sativa, Zeamays, moss: Physcomitrella patens, algae Chlorella vulgaris, Dunaliella salina,marine diatoms.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 1000 (WB).

Reinheit: Affinity purified serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Detection of NR in protein extracts from leaves of Columbia (1) and NR knockout(2-3) mutants of Arabidopsis thaliana. Each lane was loaded with the protein contained in2mg fresh weight leaf tissue (~15ug total protein). Mutants contained 56% (2) and 5% (3)of the NR activity of Columbia. Proteins were extracted in 0.1M Hepes pH 7.5, 1mM EDTA,0.1% Triton X-100, 2 mM dithiothreitol, 0.7% Protease cocktail mix for plants (Sigma), andseparated on 12% SDS-PAGE and blotted for 1h to nitrocellulose. Filters were blocked for1h with 2% BSA in TBS-T (0.1% TWEEN 20) and probed with anti-NR antibodies AS08310 in dilution 1:1000, 1h) incubation time and secondary anti-rabbit (1: 4 000, 1 h)antibody (HRP conjugated, DAKO) in TBS-T containing 2% BSA. Antibody incubationswere followed by three washings in TBS-T (15 min each). All steps were performed at RTwith agitation. Signal was detected on blue light sensitive film (exposure time 1 min) withstandard ECL (GE Healthcare). The image was captured with GS800 calibrateddensitometer (BioRad, UK Ltd). Black arrow shows gel origin, blue arrows show bands ofcross reactivity. Courtesy Dr. Alison Kingston-Smith and Teri Davies, Aberystwyth University 1

anti-Nitrate reductase, assimilatory (NR) antibody (Image 2)

anti-Nitrate reductase, assimilatory (NR) antibody (Image 3)