D1 Protein of PSII, C-terminal (PsbA) Antikörper

Antigen: D1 Protein of PSII, C-terminal (PsbA)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Proso millet (Panicum miliaceum), Guinea gras (Panicum maximum), Maize/Corn (Zea mays), Moss (Physcomitrella patens), Chlamydomonas reinhardtii (C. reinhardtii), Synechococcus PCC 7942, Cyanobacterium Anabaena sp. PCC 7120, Prochlorococcus, Zooxanthellae (Symbiodinium)

Applikation: Western Blot (WB)

Produktnummer: ABIN334536

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from available plant,algal andcyanobacterial PsbA sequences, including Arabidopsis thaliana AtCg00020,Oryza sativa P0C434, Physcomitrella patens Q6YXN7, Chlamydomonasreinhardtii P07753, Synechococcus sp. P14660

Format: Lyophilized

Beschreibung: The psbA gene has been cloned from many species of plants, green algae, andcyanobacteria. The psbA gene is located in the chloroplast genome and encodesfor the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycledunder illumination in all oxygenic photobionts. Tracking PsbA pools using theGlobal PsbA antibody can show the functional content of Photosystem II in a widerange of samples.

Spezifität: Predicted reactivity: di and monocots,conifers, brown and red algae, cyanobacteria, cellular[compartment marker] of thylakoid membrane.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 10 000 with standard ECL (WB). Additional Information: A number of degradation products may be observed when using anti-PsbAantibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cellsprior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three differentbands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors orbreakdown product.

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Brown, Campbell, Lawrence: "Resource dynamics during infection of Micromonas pusilla by virus MpV-Sp1." in: Environmental microbiology, Vol. 9, Issue 11, pp. 2720-7, 2007 (PubMed).