Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1) Antikörper

Antigen: Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1)

Synonyme: OF, PU.1, SFPI1, SPI-1, SPI-A

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN334471

Produktbeschreibung

Weitere Bezeichnung: PU.1

Gen-ID: 6688, 366126 (rat)

Immunogen: C-DLYQRQTHEYY

Beschreibung: All names and symbols: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1. Official Symbol: SPI1. RefSeq number(s): NP_001074016.1, NP_003111.2

Spezifität: This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2). Human, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Harendza S et al, J Biol Chem. 2000 Jun 30,275(26):19552-9., PMID: 10867017). Recommended concentration: 0.03-0.1µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN334471 (0.03µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Uvarov, Ludwig, Markkanen et al.: "Upregulation of the neuron-specific K+/Cl- cotransporter expression by transcription factor early growth response 4." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 52, pp. 13463-73, 2006 (PubMed).