SPI1 Antikörper (Internal Region)

Produktnummer ABIN334471

Dieser Ziege Polyklonal Antikörper detektiert spezifisch SPI1 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SPI1 Antikörper (Internal Region) (ABIN334471)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SPI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-SPI1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: PU.1

Sequenz: DLYQRQTHEY Y

Spezifität: This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).

Kreuzreaktivität: Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLYQRQTHEYY, from the internal region of the protein sequence according to NP_001074016.1, NP_003111.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2 kDa according to NP_001074016.1 and of 31.1 kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with differe

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Andere Bezeichnung: SPI1

Hintergrund: PU.1, SPI1, spleen focus forming virus (SFFV) proviral integration oncogene spi1 , hCG_25181, SFPI1, SPI-1, SPI-A, 31 kDa transforming protein, SPI-1 proto-oncogene, hematopoietic transcription factor PU.1

Gen-ID: 6688, 366126

NCBI Accession: NP_001074016, NP_003111

Pathways: Stem Cell Maintenance