Poly(rC) Binding Protein 4 (PCBP4) Antikörper

Antigen: Poly(rC) Binding Protein 4 (PCBP4)

Synonyme: LIP4, MCG10

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN334457

Produktbeschreibung

Weitere Bezeichnung: PCBP4

Gen-ID: 57060, 59092 (mouse), 363133 (rat)

Immunogen: C-NGSKKAERQKFSPY

Beschreibung: All names and symbols: PCBP4, poly(rC) binding protein 4 , LIP4, MCG10 , LYST-interacting protein, RNA binding protein MCG10, alphaCP-4 protein, poly(rC)-binding protein 4, alpha CP-4,. Official Symbol: PCBP4. RefSeq number(s): NP_065151.1, NP_127501.1, NP_127502.1, NP_127503.1

Spezifität: This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1). Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx. 40kDa band observed in lysates of cell line HeLa (calculated MW of 41.4kDa according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN334457 (0.03µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing PCBP4 (RC200749) and probed with ABIN334457 (mock transfection in first lane), tested by Origene.

Publikationen

Publikationen: Pio, Zudaire, Pino et al.: "Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer." in: Cancer research, Vol. 64, Issue 12, pp. 4171-9, 2004 (PubMed).