P2RX4 Antikörper (C-Term)

Produktnummer ABIN334455

Zitiert in 2+ Publikationen. Der Ziege Polyklonal anti-P2RX4 Antikörper (ABIN334455) detektiert spezifisch P2RX4 in WB, ELISA und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für P2RX4 Antikörper (C-Term) (ABIN334455)

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser P2RX4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-P2RX4 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: P2X4 / P2X4R

Sequenz: YREKKYKYVE DYEQ

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YREKKYKYVEDYEQ, from the C Terminus of the protein sequence according to NP_002551.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in Human Kidney lysates and in preliminary testing of Human Liver lysate (calculated MW of 43.4 kDa according to NP_002551.2).This molecular weight is routinely observed by other sources and was successfully blocke

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-P2RX4 Antikörper (ABIN334455)

Freeman, Bowman, Zetter: "Regenerative protein thymosin beta-4 is a novel regulator of purinergic signaling." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 3, pp. 907-15, (2011) (PubMed).

Yuan, Zhu, Zhou, Chen, Zhu, Ma, He, Tian, Shi: "Role of mast cell activation in inducing microglial cells to release neurotrophin." in: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).

Details zu P2RX4

Target: P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))

Andere Bezeichnung: P2RX4

Hintergrund: P2X4, P2X4R, P2RX4, purinergic receptor P2X, ligand-gated ion channel, 4, ATP receptor, ATP-gated cation channel protein, P2X purinoceptor 4, P2X receptor, subunit 4, purinergic receptor P2X ligand-gated ion channel 4, purinergic receptor P2X4, purinocept

Gen-ID: 5025, 18438, 29659

NCBI Accession: NP_002551

Pathways: Synaptic Membrane