Acylglycerol Kinase (AGK) Antikörper

Antigen: Acylglycerol Kinase (AGK)

Synonyme: MULK, FLJ10842, Mulk, AI465370, 2610037M15Rik, 6720408I04Rik, mulk, fi38e09, zgc:55462, wu:fi38e09, MGC52920, RGD1562046, MGC157181, DKFZp459M1943

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN334340

Produktbeschreibung

Weitere Bezeichnung: Acylglycerol kinase

Gen-ID: 55750, 69923 (mouse)

Immunogen: CDPRKREQMLTSP

Beschreibung: All names and symbols: acylglycerol kinase, FLJ10842 , MULK, multi-substrate lipid kinase , multiple substrate lipid kinase, AGK. Official Symbol: AGK. RefSeq number(s): NP_060708.1

Spezifität: Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 50kDa band observed in Human Brain (Substantia nigra, Hippocampus) lysates (calculated MW of 47.1kDa according to NP_060708.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN334340 (1µg/ml) staining of Human Brain (Substantia nigra) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Outeiro, Kontopoulos, Altmann et al.: "Sirtuin 2 inhibitors rescue alpha-synuclein-mediated toxicity in models of Parkinson's disease." in: Science (New York, N.Y.), Vol. 317, Issue 5837, pp. 516-9, 2007 (PubMed).