Lipase, Endothelial (LIPG) Antikörper

Antigen: Lipase, Endothelial (LIPG)

Synonyme: EL, EDL, PRO719, MGC55345, zgc:55345, MGC69106, LIPG, lipe

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN308446

Produktbeschreibung

Weitere Bezeichnung: Endothelial lipase

Gen-ID: 9388

Immunogen: C-RFNLRTSKDPEHEG

Format: Affinity-purified

Beschreibung: All names and symbols: endothelial lipase, lipase, endothelial, LIPG, EDL, EL, PRO719, endothelial cell-derived lipase, lipoprotein lipase H. Official Symbol: LIPG. RefSeq number(s): NP_006024.1

Spezifität: Human

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 70kDa band observed in Human Liver lysates (calculated MW of 56.8kDa according to NP_006024.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Gauster et al , J Clin Endocrinol Metab. 2007 Jun,92(6):2256-63. Epub 2007 Mar 13., PMID: 17356047). Recommended concentration: 0.3-1µg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN308446 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Jin, Wang, Millar et al.: "Hepatic proprotein convertases modulate HDL metabolism." in: Cell metabolism, Vol. 6, Issue 2, pp. 129-36, 2007 (PubMed).