Dpm1 (mouse) Antikörper

Antigen: Dpm1 (mouse)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Ratte (Rattus)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN308442

Produktbeschreibung

Gen-ID: 13480 (mouse), 296394 (rat)

Immunogen: PQGRSSRQDKYS-C

Format: Affinity-purified

Beschreibung: All names and symbols: DPM1, dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit, RP23-391M18.1, AI118379, AI194292, OTTMUSP00000017164, dolichol monophosphate mannose synthase, dolichyl-phosphate mannosyltransferase polypeptide 1. Official Symbol: Dpm1. RefSeq number(s): NP_034202.1

Spezifität: Mouse, Rat

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 29kDa band observed in Rat Liver lysates (calculated MW of 29.2kDa according to Mouse NP_034202.1 and to Rat NP_001100014.1). Recommended concentration: 0.1-0.3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN308442 (0.05µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Ashida, Maeda, Kinoshita: "DPM1, the catalytic subunit of dolichol-phosphate mannose synthase, is tethered to and stabilized on the endoplasmic reticulum membrane by DPM3." in: The Journal of biological chemistry, Vol. 281, Issue 2, pp. 896-904, 2006 (PubMed).