CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae) (SEPT7) (internal) Antikörper

Antigen: CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae) (SEPT7)

Synonyme: CDC3, CDC10, SEPT7A, Nbla02942

Epitope: internal

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN308440

Produktbeschreibung

Weitere Bezeichnung: Septin-7 / SEPT7 (Internal)

Gen-ID: 989, 235072 (mouse), 64551 (rat)

Immunogen: C-DNNKNKGQLTKSP

Format: Affinity-purified

Beschreibung: All names and symbols: SEPT7, septin 7, CDC10, CDC3, Nbla02942, SEPT7A, CDC10 (cell division cycle 10, S. cerevisiae, homolog), CDC10 cell division cycle 10 homolog, cell division cycle 10. Official Symbol: 01.09.07. RefSeq number(s): NP_001779.3, NP_001011553.2

Spezifität: This antibody is expected to recognize both reported isoforms (NP_001779.3, NP_001011553.2). Human, Mouse, Rat, Cow, Horse, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7kDa according to NP_001779.3 and of 55.6kDa according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN308440 (0.5µg/ml) staining of nuclear HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Kremer, Adang, Macara: "Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7." in: Cell, Vol. 130, Issue 5, pp. 837-50, 2007 (PubMed).