Clathrin, Light Chain A/B (CLTA/CLTB) Antikörper

Antigen: Clathrin, Light Chain A/B (CLTA/CLTB)

Synonyme: LCA, AV026556, CLTA, CLTA2, MGC140767, lca, MGC75712, zgc:73358, wu:fb61d08, wu:fj48d06, CLTB, clc, clcb

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN308426

Produktbeschreibung

Weitere Bezeichnung: CTLA + CTLB

Gen-ID: 1211, 12, 12757 (mouse)

Immunogen: CDFNPKSSKQAKD

Format: Affinity-purified

Beschreibung: All names and symbols: CLTA, CTLB, clathrin, light chain (Lca), LCA, clathrin, light polypeptide (Lca), clathrin, light polypeptide A, clathrin, light chain (Lcb), LCB. Official Symbol: CLTA. RefSeq number(s): NP_001824.1, NP_009027.1, NP_001070145.1, NP_001825.1, NP_009028.1

Spezifität: This antibody is expected to recognize all reported isoforms of light chains a and b. Human, Mouse, Rat, Dog, Cow

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep,279(3):C833-51., PMID: 10942733). Recommended concentration: 0.1-0.3µg/ml.

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN308426 (0.1µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Poupon, Girard, Legendre-Guillemin et al.: "Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 168-73, 2008 (PubMed).