COL18A1 Antikörper

Produktnummer ABIN307188

Der Maus Monoklonal anti-COL18A1 Antikörper (ABIN307188) detektiert spezifisch COL18A1 in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für COL18A1 Antikörper (ABIN307188)

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COL18A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-COL18A1 Antikörper

Spezifität: Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.

Aufreinigung: Thiophilic adsorption

Immunogen: Full-length recombinant murine endostatin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:100), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50 ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 8.0, 0.05 % sodium azide, before the addition of glycerol to 30 %. Sourced in Ascites.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Andere Bezeichnung: COL18A1 / Endostatin

Substanzklasse: Chemical

Hintergrund: Name/Gene ID: COL18A1
Family: Collagen

Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1

Gen-ID: 80781

UniProt: P39060