PRKG1 Antikörper (AA 657-671)

Produktnummer ABIN307178

Der Kaninchen Polyklonal anti-PRKG1 Antikörper wird verwendet zum Nachweis von PRKG1 in Proben von Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Hund, Fledermaus, Pferd, Affe und Kaninchen. Er wurde validiert für WB, IHC und IHC (p).

Kurzübersicht für PRKG1 Antikörper (AA 657-671) (ABIN307178)

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Huhn, Hund, Fledermaus, Pferd, Affe, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PRKG1 Antikörper

Bindungsspezifität: AA 657-671

Spezifität: Recognizes human PKG1 alpha at ~75kD.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Pufferfish (93%) Xenopus, Zebrafish (87%) Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Aufreinigung: Protein A purified

Immunogen: A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Pufferfish (93%), Xenopus, Zebrafish (87%), Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PRKG1

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Andere Bezeichnung: PKG / PRKG1

Hintergrund: Name/Gene ID: PRKG1
Subfamily: CGMP
Family: Protein Kinase

Synonyms: PRKG1, CGKI, CGMP kinase I, CGMP protein kinase type I, CGK-I, CGK1, PKGI, PKG I, PRKGR1B, PRKG1B, PRKGR1A, PGK, PKG, CGK, CGK 1, CGKI-alpha, CGKI-BETA

Gen-ID: 5592

UniProt: Q13976